An experimental study of the effect of autologous platelet rich plasma on the healing process of tendon in donkeys

The aim of this study was to evaluate the regenerative effect of platelets rich plasma PRP on tendon healing in donkeys. Eight adult donkeys from both sexes were used. The age range from 2-4 years and weighting 150-180 kg. The animals divided into two groups control and treatment group each group consists of four animals. The donkeys in both groups were given deep narcosis with 10% chloral hydrate at a dose of 5 g ⁄ 50 kg of body weight I.V after the premedication with 2% xylazine at a dose of 1.1mg/kg of body weight I.V. The superficial flexor digital tendon of forelimb was severed transversely and sutured by bunnell-Mayer technique using stainless steel suture and the limb was totally immobilized with plaster of paris. In the second group donkeys underwent the same procedures with topical application of platelets rich plasma on the site of sectioned tendon. At 30 post operative days the results revealed that there was a noticeable improvement of the functional activity of the injured tendon treated with platelets rich plasma PRP. Histologically there was granulation tissue at the site of healing of sectioned tendon. We conclude that platelets rich plasma lead to functional improvement of the injured tendon.